What is the purpose of humane endpoints in laboratory studies?

• A. To prevent or minimize pain and distress by terminating the experiment or initiating rescue or treatment when predefined criteria are met.
• B. To maximize data collection at all costs.
• C. To ensure animals are never withdrawn from the study.
• D. To reduce costs by shortening experiments.

Answer: (B)

Humane endpoints are predefined criteria used to prevent or minimize pain and distress in animals participating in research. When an animal meets these criteria—such as significant weight loss, severe or unrelieved pain, inability to eat or drink, or signs indicating a poor prognosis—the study for that animal is terminated or rescue/treatment is initiated. This approach protects welfare, fulfills ethical and regulatory obligations, and helps ensure that data are collected without causing unnecessary suffering. Endpoints are set in advance with veterinary input and may include euthanasia when recovery is unlikely. They are about safeguarding welfare, not maximizing data or reducing costs.