What is the purpose of endpoints in an IACUC protocol?

• A. To schedule the next annual review.
• B. To determine the funding source.
• C. To specify the timepoints for data collection.
• D. To define humane endpoints signaling when animals should be removed from the study or euthanized to prevent unnecessary pain or distress.

Answer: (D)

End points in an IACUC protocol establish humane criteria for when an animal should be removed from a study or euthanized to prevent unnecessary pain or distress. They are predetermined, objective triggers—such as a certain amount of weight loss, signs of severe pain or distress, inability to eat or move normally, or a predefined tumor burden—that, when met, prompt early termination to protect welfare. Having clear humane endpoints guides timely, compassionate decisions and helps ensure compliance with animal welfare standards while preserving the integrity of the science. The other options describe administrative scheduling, funding decisions, or data collection timing, none of which address when animal welfare is compromised and action is needed.